am indicates the apical meristem, fl indicates the developing florets, gl indicates glume primordia.Įxpression of the P VRN1:GFP and P VRN1EX1-2::GFP constructs in control versus cold treated seedlings. (E,F) Fluorescence in the cytoplasm of cells in the developing leaves of 18 day old plants. (C,D) Shoot apices at different stages of reproductive development. (B) The shoot apex at the late vegetative stage of 18 day old plants. (A) GFP fluorescence in the vegetative shoot apex of 12 day old transgenic plants carrying the P VRN1:GFP construct. GFP fluorescence in the leaves and shoot apices of transgenic plants with the P VRN1:GFP construct. Arrow indicates transcriptional start site, which did not vary between control or prolonged cold treatments. Scale indicates base pairs from the translational start site of MADS box open reading frame, indicated as negative relative to the coding regions of the VRN1 locus. (B) The P VRN1EX1-2:GFP construct was generated by fusing the same promoter region to exon1 and exon 2 of VRN1 (EX1 and EX2), separated by a 0.35 kb segment of intron 1, followed by the GFP-NOS cassette. (A) A 2.0 kb fragment (SpeI-NcoI) from the VRN1 promoter was fused to the GFP reporter gene with the NOPALINE SYNTHASE (NOS) terminator sequence to generate the P VRN1:GFP construct. Schematic representation of VRN1reporter gene constructs.
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